Evaluation of salt-soluble protein extract from an ancient wheat progenitor (<i>Aegilops tauschii</i>) for intrinsic allergenicity in an adjuvant-free mouse model of wheat allergy

Abstract Wheat allergies are a growing significant health concern around the globe. The ancient diploid wheat progenitor, Aegilops tauschii (genome DD), contributed to genetic diversity of common soft white (Triticum aestivum, genomes AABBDD). allergenic potential Ae. is largely unknown. Here we tested hypothesis that salt-soluble protein extract (SSPE) from will be intrinsically in an adjuvant-free mouse model allergy. progenitor was grown at Michigan State University greenhouse using seeds stored our breeding program. SSPE prepared, characterized for quality, and then uses skin sensitization followed by oral allergen challenge. Balb/c mice were produced maintained on plant protein-free diet. Groups adult (n=10/group) repeatedly exposed or vehicle via skin. Allergic assessed specific IgE (sIgE) response. Oral anaphylaxis quantified hypothermic shock response (HSR). mucosal mast cell (MMCR) measuring MMCP-1 blood. Repeated exposure but not elicited robust sIgE challenge significant, modest HSR as well MMCR. In summary, report characterization intrinsic allergenicity obtained first time. Funding: USDA/NIFA. United States Department Agriculture (USDA)/National Institute Food (NIFA); Hatch project MICL02486 (Accession Number: 1012322); MICL01699; Agricultural Research Initiative Competitive Program, grant number: 2018-67017-27876